Chii chinonzi machubhu ekuunganidza ropa eEDTA uye anoshandiswa sei?

Mukuongorora kwechiremba uye kuongororwa kwekiriniki nekurapwa,Machubhu ekuunganidza ropa eEDTA, sezvinhu zvakakosha pakuunganidza ropa, zvinoita basa rakakosha mukusimbisa kusimba kwemasampuli uye kururama kwekuongororwa. Muchinyorwa chino, tichaongorora zvakakwana "muchengeti asingaonekwe" uyu mune zvehutano kubva muzvinhu zvetsanangudzo, kupatsanurwa kwemavara, musimboti wekudzivirira ropa kupwanya ropa, chinangwa chekuongorora uye mwero wekushandiswa.

ChiiChubhu yekuunganidza ropa yeEDTA?

Chubhu yekuunganidza ropa yeEDTA rudzi rwechubhu yekuunganidza ropa ine Ethylene Diamine Tetraacetic Acid kana munyu wayo, iyo inonyanya kushandiswa kuunganidza sampuro dzeropa uye kurapa anticoagulant. EDTA inogona kudzivirira coagulation cascade reaction nekubvisa calcium ions muropa, kuitira kuti ropa rirambe riri mumvura kwenguva yakareba, uye kupa sampuro dzakagadzikana dzekuedzwa kweropa uye molecular biology. Inopa sampuro dzakagadzikana dzeropa, molecular biology nedzimwe bvunzo.

Sechikamu chakakosha chezvinodyiwa zvekurapa, machubhu ekuunganidza ropa eEDTA anofanirwa kutevedzera mwero wenyika we "Zvirongo zvekuunganidza sample yeropa zvinoshandiswa kamwe chete" (semuenzaniso GB/T 19489-2008) kuti ive nechokwadi chekuti hapana hutachiona, hapana hutachiona uye hapana chepfu.

Mavara akasiyana emachubhu ekuunganidza ropa eEDTA

Zvichienderana nezvinodiwa pasi rose (zvakadai seCLSI H3-A6 nhungamiro), machubhu ekuunganidzira ropa eEDTA anowanzo vharwa nepepuru (EDTA-K2/K3) kana bhuruu (sodium citrate yakasanganiswa neEDTA) kuti pave nekusiyana kwekushandiswa:

Cherechedza: Mamwe marudzi anogona kunge aine mamwe mavara, tarisa mirairo usati washandisa.

Maitiro ekudzivirira ropa kubva muchubhu dzeEDTA dzinounganidza ropa

EDTA kuburikidza neboka rayo remamorekuru carboxyl (-COOH) uye ma calcium ions muropa (Ca²⁺) zvakabatana kuti zvigadzire chelate yakagadzikana, nokudaro zvichidzivisa kushanda kwe plasminogen, zvichivhara maitiro ekugwamba kwe fibrinogen kuita fibrin. Kurwisa kugwamba uku kune hunhu hunotevera:

1. Kukurumidza kushanda: mushonga wekudzivirira ropa kuti risaungane unogona kupedzwa mukati memaminitsi 1-2 mushure mekutorwa kweropa;

2. kugadzikana kwakanyanya: mienzaniso inogona kuchengetwa kwemaawa anopfuura makumi mana nemasere (mufiriji inogona kuwedzerwa kusvika maawa makumi manomwe nemaviri);

3. Kushandiswa kwakasiyana-siyana: kwakakodzera bvunzo zhinji dzeropa, asi kwete bvunzo dzema "coagulation" kana "platelet function" (machubhu e sodium citrate anodiwa).

Zvinhu zvekuongorora ropa zveEDTA tube

1. kuongorora ropa nguva dzose: huwandu hwemasero machena eropa, zviyero zvemasero matsvuku eropa, huwandu hwehemoglobin, nezvimwewo;

2. Kuzivikanwa kweboka reropa uye kuenzaniswa kwaro: boka reropa reABO, kuonekwa kweRh factor;

3. kuongorora mamorekuru: kuongororwa kwemajini, kuona huwandu hwehutachiona (semuenzaniso HIV, HBV);

4. hemoglobin ine glycated (HbA1c): kuongororwa kwenguva refu kweshuga muropa nekuda kwechirwere cheshuga;

5. Kuongororwa kwezvirwere zveropa: Plasmodium, kuonekwa kwe microfilariae.

Kushandisa mitemo nematanho ekudzivirira

1. Maitiro ekuunganidza:

Mushure mekuchenesa ganda nehutachiona, shandai zvinoenderana nemutemo wekuunganidzwa kweropa mutsinga;

Mushure mekutorwa kweropa, shandura chubhu yekuunganidzira ropa ka5-8 kuti uve nechokwadi chekuti mushonga unorwisa ropa wasanganiswa zvakakwana neropa;

Dzivisa kuzununguka zvine simba (kudzivirira kubuda kweropa).

2. Kuchengeta nekutakura:

Chengeta patembiricha yemumba (15-25°C), usapise kana kutonhora;

Isa yakatwasuka panguva yekufambisa kudzivirira kusunungura kwechubhu.

3. mamiriro ezvinhu asingabvumirwi:

Machubhu eSodium citrate anodiwa kuti pave neCoagulation IV (PT, APTT, nezvimwewo);

Kuongororwa kwebasa remaplatelet kunoda chubhu ye sodium citrate.

Maitiro ekusarudza mhando yepamusoroChubhu yekuunganidza ropa yeEDTA?

1. Chitupa uye chitupa: sarudza zvigadzirwa zvakapasa ISO13485 uye chitupa cheCE. 2;

2. Kuchengetedzwa kwezvinhu: muviri wechubhu unofanira kujeka uye usina plasticizer residues;

3. Kuyera kwakarurama: huwandu hwemushonga unorwisa ropa hunowedzerwa hunofanira kuenderana nezvinodiwa nenyika (semuenzaniso huwandu hweEDTA-K2 hwe1.8±0.15mg/mL);

4. Mukurumbira wechiratidzo: Makambani anozivikanwa mune zvekurapa anopiwa mukana wekuti zvinhu zvigare zvakanaka.

Mhedziso

Semunhu akakosha wemudziyo wekuunganidza ropa, machubhu ekuunganidza ropa eEDTA ane simba rakananga pakururama kwemhedzisiro yekuyedzwa maererano nehunhu hwawo hweanticoagulant. Nekugadzirisa kushandiswa kwemachubhu ekuunganidza ropa akasiyana-siyana ane mavara uye kuasanganisa nemaitiro akasimba ekuunganidza, zvinogona kupa hwaro hwakavimbika hwekuongororwa kwekiriniki. Mune ramangwana, nekuvandudzwa kwemishonga chaiyo, machubhu ekuunganidza ropa eEDTA achaita basa rakakosha mukuongorora ropa, kutevedzana kwemajini nedzimwe nzvimbo, uye acharamba achichengetedza hutano hwevanhu.