Chii chinonzi EDTA Ropa rekuunganidza Machubhu uye Anoshandiswa Sei?

Mukuedzwa kwekurapa uye kuongororwa kwekiriniki uye kurapwa,EDTA machubhu ekuunganidza ropa, sezvinonyanya kushandiswa pakuunganidza ropa, vanoita basa rinokosha mukuvimbisa kuvimbika kwemasampuli uye nekururama kwekuedzwa. Muchinyorwa chino, isu tichaongorora zvizere uyu "asingaoneki muchengeti" munharaunda yekurapa kubva kuzvinhu zvetsanangudzo, kupatsanurwa kwemavara, anticoagulation musimboti, kuyedza chinangwa uye chiyero chekushandisa.

ChiiEDTA yekuunganidza ropa chubhu?

EDTA yekuunganidza ropa chubhu imhando yevacuum yekuunganidza ropa chubhu ine Ethylene Diamine Tetraacetic Acid kana munyu wayo, unonyanya kushandiswa kuunganidzwa kweropa uye anticoagulant kurapwa. EDTA inogona kuvharidzira coagulation cascade reaction nekucheka ma calcium ions muropa, kuitira kuti ropa rirambe riri mumhepo kwenguva yakareba, uye kupa sampuli dzakagadzikana dzekuongororwa kweropa uye molecular biology. Inopa sampuli dzakagadzikana dzemaitiro eropa, molecular biology uye kumwe bvunzo.

Sechikamu chakakosha chemishonga inodyiwa, Machubhu ekuunganidza ropa eEDTA anofanirwa kutevedzera chiyero chenyika che "Single-use venous blood sample collection containers" (eg GB/T 19489-2008) kuti ive nechokwadi chekushanda kwe sterility, non-pyrogenic uye non-cytotoxicity.

Akasiyana mavara eEDTA ekuunganidza ropa machubhu

Zvinoenderana nezviyero zvepasi rese (senge CLSI H3-A6 nhungamiro), machubhu ekuunganidza ropa eEDTA anowanzo kuvharirwa nepepuru (EDTA-K2/K3) kana bhuruu (sodium citrate yakasanganiswa neEDTA) kusiyanisa kushandiswa:

Ongorora: Mamwe mabhureki anogona kunyorwa mune mamwe mavara, tarisa mirairo usati washandisa.

Anticoagulation maitiro eEDTA ekuunganidza ropa machubhu

EDTA kuburikidza neayo molecular carboxyl boka (-COOH) uye calcium ions muropa (Ca²⁺) yakasanganiswa kuti iite chelate yakagadzikana, nokudaro ichidzivisa kushandiswa kweplasminogen, ichivharira coagulation process ye fibrinogen mu fibrin. Iyi anticoagulation ine zvinotevera maitiro:

1. kukurumidza kutanga kwechiito: anticoagulation inogona kupedzwa mukati memaminitsi 1-2 mushure mekuunganidza ropa;

2. kugadzikana kwepamusoro: samples inogona kuchengetwa kwemaawa anopfuura 48 (firiji inogona kuwedzerwa kusvika maawa 72);

3. Yakasiyana-siyana yekushandiswa: inokodzera kuongororwa kwakawanda kwehematology, asi kwete yekubatanidza kana kuti platelet function tests (sodium citrate tubes inodiwa).

Zvinhu zvekuongorora zveEDTA zvekuunganidza ropa

1. Kuongororwa kweropa nguva dzose: masero machena eropa, masero matsvuku eropa, hemoglobin concentration, nezvimwewo;

2. Kuzivikanwa kweboka reropa uye kuyambuka-kufananidza: ABO boka reropa, Rh factor kuona;

3. molecular diagnosis: genetic test, viral load determination (eg HIV, HBV);

4. glycated hemoglobin (HbA1c): yenguva refu yeropa glucose kutarisa kune chirwere cheshuga mellitus;

5. kuongororwa kweparasite yeropa: Plasmodium, microfilariae kuonekwa.

Kushandiswa kwemaitiro uye zvidziviriro

1. Maitiro ekuunganidza:

Mushure mekuuraya utachiona paganda, shanda zvinoenderana nechiyero chekuunganidza ropa re venous;

Pakarepo mushure mekuunganidza, inverted the blood collection tube 5-8 nguva kuti uone kuti anticoagulant yakanyatsosanganiswa neropa;

Dzivisa kudengenyeka kwechisimba (kudzivirira hemolysis).

2. Kuchengeta uye zvekufambisa:

Chengetedza panzvimbo yekushisa (15-25 ° C), usapisa kana kupisa;

Isa vertically panguva yekufambisa kudzivirira kusunungurwa kwechubhu chivharo.

3. Contraindication mamiriro:

Sodium citrate tubes inodiwa paCoagulation IV (PT, APTT, nezvimwewo);

Platelet function test inoda sodium citrate chubhu.

Nzira yekusarudza yakakwiriraEDTA yekuunganidza ropa chubhu?

1. Kufanirwa uye chitupa: sarudza zvigadzirwa zvakapfuura ISO13485 uye CE certification. 2;

2. Kuchengetedzwa kwezvinhu: muviri wechubhu unofanirwa kuve wakajeka uye usina masara epurasitiki;

3. Yakarurama dosing: chiyero che anticoagulant chakawedzerwa chinofanira kunge chichienderana nehuwandu hwenyika (eg EDTA-K2 concentration ye 1.8± 0.15mg / mL);

4. Kuzivikanwa kwechigadzirwa: Chinonyanya kupiwa kune mazita anozivikanwa mumunda wezvigadzirwa zvemishonga kuti zvive nechokwadi chekugadzikana kwebatch.

Mhedziso

Senhengo yakakosha yemudziyo wekuunganidza ropa, EDTA machubhu ekuunganidza ropa ane mhedzisiro yakananga pakurongeka kwemhedzisiro yebvunzo maererano neiyo anticoagulant maitiro. Nekugadzirisa kushandiswa kwemachubhu ekuunganidza ropa akasiyana-siyana ane mavara uye nekuabatanidza nemaitiro akasimba ekuunganidza, zvinogona kupa hwaro hwakavimbika hwekuongororwa kwekiriniki. Mune ramangwana, nekugadzirwa kwemushonga chaiwo, machubhu ekuunganidza ropa eEDTA achaita basa rakakosha pakuongorora ropa, kutevedzana kwemajini nemimwe minda, uye kuenderera mberi nekuchengetedza hutano hwevanhu.